Journal: American Journal of Cancer Research

Article Title: Overexpression of MEF2D contributes to oncogenic malignancy and chemotherapeutic resistance in ovarian carcinoma

doi:

Figure Lengend Snippet: Analyses of MEF2D expression in OC cell lines and patient samples. A. Data on MEF2D DNA copy number and mRNA expression in ovarian cancer and normal ovarian tissue from several study groups deposited in the Oncomine database (www.oncomine.org). B. The protein expression of MEF2D in normal human ovarian epithelial cells (IOSE) and human OC cells (OVCAR3, SKOV3) was determined by western blot assays. C. The protein expression of MEF2D in normal ovarian tissues (N1-N4) and human OC tissues (T1-T12) was determined by western blot assays. Error bars represent the s.d. of triplicate measurements. *P < 0.05; **P < 0.01; ***P < 0.001. D. IHC analysis of MEF2D protein expression in normal ovarian tissues, and OC tissues Original magnifications: × 200 and × 400. E. IHC analysis of IKBKE and HPSE protein expression in normal ovarian tissues, and OC tissues Original magnifications: × 200 and × 400.

Article Snippet: Cell lines culture and establishment of DDP resistant cells Human ovarian cancer OVCAR3, SKOV3 cell lines and normal human ovarian epithelial cells IOSE were obtained from Sciencell (California, USA), and authenticated by short tandem repeat.

Techniques: Expressing, Western Blot

Journal: American Journal of Cancer Research

Article Title: Overexpression of MEF2D contributes to oncogenic malignancy and chemotherapeutic resistance in ovarian carcinoma

doi:

Figure Lengend Snippet: Effect of MEF2D knockdown on proliferation and apoptosis of OC cells in vitro. A. CCK-8 assays were performed to determine the effects of MEF2D knockdown on the proliferation of SKOV3 and OVCAR3 cells. Cell viability was determined at 0, 12, 24, 48 and 72 h. B. Flowcytometry assays were performed to determine the effects of MEF2D knockdown on the apoptosis of SKOV3 and OVCAR3 cells. C. Effects of MEF2D knockdown on proliferation-associated protein cyclin-D1 and c-myc and apoptosis-related protein caspase3 and cleaved caspase3 were analyzed by western blotting in SKOV3 and OVCAR3 cells. Error bars represent the s.d. of triplicate measurements. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Cell lines culture and establishment of DDP resistant cells Human ovarian cancer OVCAR3, SKOV3 cell lines and normal human ovarian epithelial cells IOSE were obtained from Sciencell (California, USA), and authenticated by short tandem repeat.

Techniques: Knockdown, In Vitro, CCK-8 Assay, Western Blot

Journal: American Journal of Cancer Research

Article Title: Overexpression of MEF2D contributes to oncogenic malignancy and chemotherapeutic resistance in ovarian carcinoma

doi:

Figure Lengend Snippet: Effect of MEF2D knockdown on invasion and migration of OC cells in vitro. A. The migration abilities of SKOV3 and OVCAR3 were measured through testing the wound closure after MEF2D knockdown using wound healing assays. B. Transwell assays were used to detect the migration and invasion abilities after MEF2D knockdown in SKOV3 and OVCAR3 cells. Original magnifications, × 200 and × 400. C. Effects of MEF2D knockdown on migration associated protein MMP9 were analyzed by western blotting in SKOV3 and OVCAR3 cells. Error bars represent the s.d. of triplicate measurements. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Cell lines culture and establishment of DDP resistant cells Human ovarian cancer OVCAR3, SKOV3 cell lines and normal human ovarian epithelial cells IOSE were obtained from Sciencell (California, USA), and authenticated by short tandem repeat.

Techniques: Knockdown, Migration, In Vitro, Western Blot

Journal: American Journal of Cancer Research

Article Title: Overexpression of MEF2D contributes to oncogenic malignancy and chemotherapeutic resistance in ovarian carcinoma

doi:

Figure Lengend Snippet: Effect of MEF2D knockdown on tumorigenecity of ovarian carcinoma cells in vivo. A, B. OVCAR3 cells were subcutaneously inoculated in SCID mice, which were randomly grouped to NC or siMEF2D001 (n = 6 for each group) and then injected with NC or siMEF2D001 every 3 days. 28 days later tumors were removed for analysis. Each tumor formed was volumed and weighted. The weight and volume of established tumors was measured and is shown in a scatter plot. C. Immunohistochemical analysis of MEF2D expression was performed on OVCAR3 tumor xenografts. The representative images are shown (with original magnification, of × 200 and × 400).

Article Snippet: Cell lines culture and establishment of DDP resistant cells Human ovarian cancer OVCAR3, SKOV3 cell lines and normal human ovarian epithelial cells IOSE were obtained from Sciencell (California, USA), and authenticated by short tandem repeat.

Techniques: Knockdown, In Vivo, Injection, Immunohistochemical staining, Expressing